The effects of green and chemically-synthesized copper oxide nanoparticles on the production and gene expression of morphinan alkaloids in Oriental poppy.

Oriental poppy (Papaver orientale L.) belonging to the Papaveraceae family, has the capacity to synthesize a wide range of benzylisoquinoline alkaloids (BIAs). This experiment was conducted to investigate the effects of green and chemical copper oxide nanoparticles (CuO NPs) elicitors on oxidative stress and the BIAs biosynthesis pathway in the cell suspension culture of P. orientale. This research shows that both green and chemical CuO NPs at concentrations of 20 mg/L and 40 mg/L, induce oxidative stress in the cell suspension of P. orientale by increasing the production of H2O2 and the activity of antioxidant enzymes. The comparison of treatments revealed that utilizing a lower concentration of CuO NPs (20 mg/L) and extending the duration of cell suspension incubation (up to 48 h) play a more influential role in inducing the expression of the BIAs biosynthesis pathway genes (PsWRKY, TYDC, SalSyn, SalR, SalAT, T6ODM, COR and CODM) and increasing the production of morphinan alkaloids (thebaine, codeine, and morphine). The overarching results indicate that the concentration of CuO NPs and the duration of cell treatment have a more significant impact than the nature of CuO NPs in inducing oxidative stress and stimulating the expression of the BIAs pathway genes.

A biocompatible NPK+Fe+Zn slow release fertilizer: synthesis and its evaluation in tomato plant growth improvement.

Slow-release fertilizers (SRFs) play an essential and necessary role in sustainable agriculture. Using slow-release and environment friendly fertilizers can increase the growth of plants and reduce the loss of nutrients. Considering the deficiency of iron (Fe) and zinc (Zn) in calcareous soils, a slow-release fertilizer was prepared based on the polymeric nanocomposite, which contains NPK, Fe, and Zn. Its potential was evaluated on tomato plant growth by conducting an experiment in a factorial completely randomized design with three replications. Two levels of salinity (2 and 5 ds m-1, two types of soil texture) clay loam and sandy loam) and five levels of fertilizers were examined in the experiment. To this, the graphene oxide-chitosan coated-humic acid@Fe3O4 nanoparticles (Fe3O4@HA@GO-Cs), and the graphene oxide-chitosan coated-ammonium zinc phosphate (AZP@GO-Cs) were used as Fe and Zn sources, respectively. Then, the optimal Fe and Zn fertilizers in the presence of urea, phosphorus, and potassium slow- release fertilizers (SRF) were investigated under greenhouse conditions. The results indicated that the best improvement in growth and nutrient uptake in plants was achieved by using the SRF. Notably, in the shoots of tomato plants, the nitrogen, phosphorus, potassium, Fe, and Zn concentration increased by 44, 66, 46, 75, and 74% compared to the control. The use of nanofertilizer can be an effective, biocompatible, and economical option to provide Fe and Zn demand in plants.

Computational Insights into the Selecting Mechanism of α-Amylase Immobilized on Cellulose Nanocrystals: Unveiling the Potential of α-Amylases Immobilized for Efficient Poultry Feed Hydrolysis.

The selection of an appropriate amylase for hydrolysis poultry feed is crucial for achieving improved digestibility and high-quality feed. Cellulose nanocrystals (CNCs), which are known for their high surface area, provide an excellent platform for enzyme immobilization. Immobilization greatly enhances the operational stability of α-amylases and the efficiency of starch bioconversion in poultry feeds. In this study, we immobilized two metagenome-derived α-amylases, PersiAmy2 and PersiAmy3, on CNCs and employed computational methods to characterize and compare the degradation efficiencies of these enzymes for poultry feed hydrolysis. Experimental in vitro bioconversion assessments were performed to validate the computational outcomes. Molecular docking studies revealed the superior hydrolysis performance of PersiAmy3, which displayed stronger electrostatic interactions with CNCs. Experimental characterization demonstrated the improved performance of both α-amylases after immobilization at high temperatures (80 °C). A similar trend was observed under alkaline conditions, with α-amylase activity reaching 88% within a pH range of 8.0 to 9.0. Both immobilized α-amylases exhibited halotolerance at NaCl concentrations up to 3 M and retained over 50% of their initial activity after 13 use cycles. Notably, PersiAmy3 displayed more remarkable improvements than PersiAmy2 following immobilization, including a significant increase in activity from 65 to 80.73% at 80 °C, an increase in activity to 156.48% at a high salinity of 3 M NaCl, and a longer half-life, indicating greater thermal stability within the range of 60 to 80 °C. These findings were substantiated by the in vitro hydrolysis of poultry feed, where PersiAmy3 generated 53.53 g/L reducing sugars. This comprehensive comparison underscores the utility of computational methods as a faster and more efficient approach for selecting optimal enzymes for poultry feed hydrolysis, thereby providing valuable insights into enhancing feed digestibility and quality.

A comprehensive review on starch: Structure, modification, and applications in slow/controlled-release fertilizers in agriculture.

This comprehensive review thoroughly examines starch's structure, modifications, and applications in slow/controlled-release fertilizers (SRFs) for agricultural purposes. The review begins by exploring starch's unique structure and properties, providing insights into its molecular arrangement and physicochemical characteristics. Various methods of modifying starch, including physical, chemical, and enzymatic techniques, are discussed, highlighting their ability to impart desirable properties such as controlled release and improved stability. The review then focuses on the applications of starch in the development of SRFs. It emphasizes the role of starch-based hydrogels as effective nutrient carriers, enabling their sustained release to plants over extended periods. Additionally, incorporating starch-based hydrogel nano-composites are explored, highlighting their potential in optimizing nutrient release profiles and promoting plant growth. Furthermore, the review highlights the benefits of starch-based fertilizers in enhancing plant growth and crop yield while minimizing nutrient losses. It presents case studies and field trials demonstrating starch-based formulations' efficacy in promoting sustainable agricultural practices. Overall, this review consolidates current knowledge on starch, its modifications, and its applications in SRFs, providing valuable insights into the potential of starch-based formulations to improve nutrient management, boost crop productivity, and support sustainable agriculture.

Improvement of tomato yield and quality using slow release NPK fertilizers prepared by carnauba wax emulsion, starch-based latex and hydrogel nanocomposite combination.

The modern agriculture is working on introducing new generation of fertilizers that apt to slow down the nutrients release to be more in synchrony with plant's need throughout growth season, enhance fertilizer performance, and decrease nutrient losses into the environment. The aim of this research was to develop an advanced NPK slow-release fertilizer (SRF) and investigate its effect on yield, nutritional and morphological responses of tomato plant (Lycopersicon esculentum Mill.) as a model crop. To this goal, three water-based bio-polymeric formulations including starch-g-poly (acrylic acid-co-acrylamide) nanocomposite hydrogel, starch-g-poly(styrene-co-butylacrylate) latex, and carnauba wax emulsion were synthesized and used for production of NPK-SRF samples. Different samples of coated fertilizers (urea, potassium sulfate, and superphosphate granules) were prepared using different ratios of latex and wax emulsion, and for phosphorus and potash (R-treatment). Moreover, some of coated fertilizers (15 and 30 wt.%) was replaced with nanocomposite hydrogel containing fertilizers, named D and H treatments, respectively. The effect of SRF samples were compared with commercial fertilizers (NPK treatment) and a commercial SRF (T treatment), on the growth of tomato in the greenhouse, at two different levels (100 and 60). The efficiency of all the synthesized formulations were higher than NPK and T treatments, and among them, H100 significantly improved the morphological and physiological characteristics of tomato. For instance, amount of residual elements (nitrogen, phosphorus and potassium) as well as micro elements of calcium, iron and zinc in tomato cultivation bed and accordingly the uptake of these elements in the roots, aerial parts and fruits were increased in the R, H, and D treatments. The highest yield (1671.54 g), highest agricultural agronomy efficiency of fertilizer, and the highest dry matter percentage (9.52%) were obtained in H100. The highest amount of lycopene, antioxidant capacity and vitamin C was also observed in H100. Nitrate accumulation in tomato fruit in the synthesized SRF samples were decreased significantly compared to NPK100, and the lowest amount was observed in H100, which was 55.24% less than NPK100. Accordingly, it is suggested that combination of natural-based nanocomposite hydrogels along with coating latexes and wax emulsions can be a successful method to synthesize efficient NPK-SRF formulations for improvement of crop growth and quality.

Synthesis of two novel bio-based hydrogels using sodium alginate and chitosan and their proficiency in physical immobilization of enzymes.

Herein, four novel and bio-based hydrogel samples using sodium alginate (SA) and chitosan (CH) grafted with acrylamide (AAm) and glycidyl methacrylate (GMA) and their reinforced nanocomposites with graphene oxide (GO) were synthesized and coded as SA-g-(AAm-co-GMA), CH-g-(AAm-co-GMA), GO/SA-g-(AAm-co-GMA), and GO/CH-g-(AAm-co-GMA), respectively. The morphology, net charge, and water absorption capacity of samples were entirely changed by switching the biopolymer from SA to CH and adding a nano-filler. The proficiencies of hydrogels were compared in the immobilization of a model metagenomic-derived xylanase (PersiXyn9). The best performance was observed for GO/SA-g-poly(AAm-co-GMA) sample indicating better stabilizing electrostatic attractions between PersiXyn9 and reinforced SA-based hydrogel. Compared to the free enzyme, the immobilized PersiXyn9 on reinforced SA-based hydrogel showed a 110.1% increase in the released reducing sugar and almost double relative activity after 180 min storage. While immobilized enzyme on SA-based hydrogel displayed 58.7% activity after twelve reuse cycles, the enzyme on CH-based carrier just retained 8.5% activity after similar runs.

Highly efficient removal of dyes from wastewater using nanocellulose from quinoa husk as a carrier for immobilization of laccase.

In this study, the synthesis of nanocellulose (NC) from an agro-waste of quinoa husks (QS) was reported for the first time. The NC nano-carrier was utilized for immobilization of a model laccase enzyme (PersiLac1) providing an innovative, green, and practical nano-biocatalyst for efficient removal of two different model dyes (malachite green (MG) and congo red (CR)) from water. This nano-biocatalyst developed a synergistic adsorption-degradation approach leading the dye molecules easily gathered near the nano-carrier by adsorption and then degraded effectively by the enzyme. Upon enzyme immobilization, the dye removals (%) were remarkably improved for both 150 mg/L of dyes (from 54% and 12%, for MG and CR, respectively, in case of the pristine NCs, to 98% and 60% for the immobilized enzyme). The immobilized PersiLac1 could decolorize the concentrated dye solutions and showed superior reusability (up to 83% dye removal after 18th runs for MG) and remarkable performance from complex real textile effluents.

Enhancing the ethanol production by exploiting a novel metagenomic-derived bifunctional xylanase/ß-glucosidase enzyme with improved ß-glucosidase activity by a nanocellulose carrier.

Some enzymes can catalyze more than one chemical conversion for which they are physiologically specialized. This secondary function, which is called underground, promiscuous, metabolism, or cross activity, is recognized as a valuable feature and has received much attention for developing new catalytic functions in industrial applications. In this study, a novel bifunctional xylanase/ß-glucosidase metagenomic-derived enzyme, PersiBGLXyn1, with underground ß-glucosidase activity was mined by in-silico screening. Then, the corresponding gene was cloned, expressed and purified. The PersiBGLXyn1 improved the degradation efficiency of organic solvent pretreated coffee residue waste (CRW), and subsequently the production of bioethanol during a separate enzymatic hydrolysis and fermentation (SHF) process. After characterization, the enzyme was immobilized on a nanocellulose (NC) carrier generated from sugar beet pulp (SBP), which remarkably improved the underground activity of the enzyme up to four-fold at 80°C and up to two-fold at pH 4.0 compared to the free one. The immobilized PersiBGLXyn1 demonstrated 12 to 13-fold rise in half-life at 70 and 80°C for its underground activity. The amount of reducing sugar produced from enzymatic saccharification of the CRW was also enhanced from 12.97 g/l to 19.69 g/l by immobilization of the enzyme. Bioethanol production was 29.31 g/l for free enzyme after 72 h fermentation, while the immobilized PersiBGLXyn1 showed 51.47 g/l production titre. Overall, this study presented a cost-effective in-silico metagenomic approach to identify novel bifunctional xylanase/ß-glucosidase enzyme with underground ß-glucosidase activity. It also demonstrated the improved efficacy of the underground activities of the bifunctional enzyme as a promising alternative for fermentable sugars production and subsequent value-added products.

Silica Magnetic Graphene Oxide Improves the Effects of Stem Cell-Conditioned Medium on Acute Liver Failure.

OBJECTIVE: Acute liver failure (ALF) is usually associated with inflammation and oxidation of hepatocytes and has high mortality and resource costs. Although mesenchymal stem cell-conditioned medium (MSC-CM) has therapeutic effects similar to MSC transplant in treating liver failure, it may not increase survival. On the other hand, graphene-based nanostructures have been proven useful in biomedicine. In this study, we investigated whether silica magnetic graphene oxide (SMGO) improved the effects of MSC-CM in protecting hepatocytes and stimulating the regeneration of damaged liver cells. MATERIALS AND METHODS: To provide a rat model of ALF, male rats were injected intraperitoneally with carbon tetrachloride (CCl4). The rats were randomly divided into six groups, namely control, sham, CCl4, MSC-CM, SMGO, and MSC-CM + SMGO. In the experimental groups, the rats received, depending on the group, 2 mL/kg body weight CCl4 and either MSC-CM with 5 × 106 MSCs or 300 µg/kg body weight SMGO or both. Symptoms of ALF appeared 4 days after the injection. All groups were compared and analyzed both histologically and biochemically 4 days after the injection. RESULTS: The results indicated that the use of SMGO enhanced the effect of MSC-CM in reducing necrosis, inflammation, aspartate transaminase, alanine aminotransferase, and alkaline phosphatase in the CCl4-induced liver failure of the rat model. Also, the expression of vascular endothelial growth factor and matrix metalloproteinase-9 (MMP-9) was significantly upregulated after treatment with SMGO. CONCLUSION: SMGO improved the hepatoprotective effects of MSC-CM on acute liver damage, probably by suppressing necrosis, apoptosis, and inflammation of hepatocytes.

Effect of hydrogel composite reinforced with natural char nanoparticles on improvement of soil biological properties and the growth of water deficit-stressed tomato plant.

Hydrogel polymers have been used to enhance water and nutrient retention in agricultural soils. The incorporation of nanoparticles to yield composite hydrogels has also gained substantial momentum over the years. The aim of the research was to investigate the effect of hydrogel-nano natural char composite (reinforced starch-based hydrogels with natural char nanoparticles) at three levels 0%, 0.3% and 0.6% (w/w) on nutritional and morphological responses of tomato plant (Lycopersicon esculentum Mill.) as well as on some soil biological properties under water-deficit stress at three levels 50% water-holding capacity (WHC) (severe stress), 75% WHC (mild stress), and 85% WHC (non-stress conditions). The different levels of nano-composite and water deficit stress significantly (P < 0.05) affected plant morpho-nutritional indices and soil microbial traits. Water-deficit stress decreased all measured parameters in this assay. However, the use of nanocomposite reduced the negative effects of water-deficit stress on tomato growth and development. The magnitude of the responses to the nanocomposite treatment depended on the concentration of applied nanocomposite and stress severity with the most positive effects on the growth (22-45% increase) and nutritional indices (P, Fe, and Zn concentration) (16-29% increase) of tomato at level 0.3% hydrogel nanocomposite and 85% WHC and on soil respiration rate (61% increase) and microbial population size ( 89% increase) at the level 0.6% hydrogel nanocomposite and 75% WHC. Accordingly, it is suggested that the application of hydrogel-nano natural char composite as a promising soil amendment, if used correctly, can be a successful method to maintain soil moisture content (improved tomato growth), plant nutrients, and soil microbial activity in the tomato growing medium.

Efficient removal of various textile dyes from wastewater by novel thermo-halotolerant laccase.

A novel thermostable/halotolerant metagenome-derived laccase (PersiLac2) from tannery wastewater was purified to remove textile dyes in this study. The enzyme was highly active over a wide temperature and pH range and maintained 73.35% of its initial activity after 30 days, at 50 °C. The effect of various metal and organic-solvent tolerance on PersiLac2 showed, retaining greater than 53% activity at 800 mM of metal ions, 52.12% activity at 6 M NaCl, and greater than 44.09% activity at 20% organic solvents. PersiLac2 manifested effective removal of eight different textile dyes from azo, anthraquinone, and triphenylmethane families. It decolorized 500 mg/L of Alizarin yellow, Carmine, Congo red and Bromothymol blue with 99.74-55.85% efficiency after 15 min, at 50 °C, without mediator. This enzyme could practically remove dyes from a real textile effluent and it displayed significant detoxification in rice seed germination tests. In conclusion, PersiLac2 could be useful in future for decolorization/detoxification of wastewater.

Application of free and immobilized novel bifunctional biocatalyst in biotransformation of recalcitrant lignocellulosic biomass.

Herein, an innovative, green, and practical biocatalyst was developed using conjugation of a novel bifunctional mannanase/xylanase biocatalyst (PersiManXyn1) to the modified cellulose nanocrystals (CNCs). Firstly, PersiManXyn1 was multi-stage in-silico screened from rumen macrobiota, and then cloned, expressed, and purified. Next, CNCs were synthesized from sugar beet pulp using enzymatic and acid hydrolysis processes, and then Fe3O4 NPs were anchored on their surface to produce magnetic CNCs (MCNCs). This hybrid was modified by dopamine providing DA/MCNCs nano-carrier. The bifunctional PersiManXyn1 demonstrated the superior hydrolysis activity on corn cob compared with the monofunctional xylanase enzyme (PersiXyn2). Moreover, the immobilization of PersiManXyn1 on the nano-carrier resulted in an improvement of the thermal stability, kinetic parameters (Kcat), and storage stability of the enzyme. Incorporation of the Fe3O4 NPs on the CNCs made magnetic nano-carrier with high magnetization value (25.8 emu/g) which exhibited rapid response toward the external magnetic fields. Hence, the immobilized biocatalyst could be easily separated from the products by a magnet, and reused up to 8 cycles with maintaining more than 50% of its original activity. The immobilized PersiManXyn1 generated 22.2%, 38.7%, and 35.1% more reducing sugars after 168 h hydrolysis of the sugar beet pulp, coffee waste, and rice straw, respectively, compared to the free enzyme. Based on the results, immobilization of the bifunctional PersiManXyn1 exhibited the superb performance of the enzyme to improve the conversion of the lignocellulosic wastes into high value products and develop the cost-competition biomass operations.

Immobilization of enzyme cocktails on dopamine functionalized magnetic cellulose nanocrystals to enhance sugar bioconversion: A biomass reusing loop.

A combined enzymatic treatment/acid hydrolysis technique was utilized to synthesize cellulose nanocrystals (CNCs) from sugar beet pulp. CNCs were functionalized with magnetite nanoparticles and dopamine making a versatile nano-carrier (DA/Fe3O4NPs@CNCs) for covalent enzyme immobilization. Oxygene/amine functionalities, high magnetization value, and specific surface area of DA/Fe3O4NPs@CNCs made it a reusable and green candidate for conjugation to hydrolytic enzyme cocktails (three cellulases, two hemicellulases, and their combinations) to prepare an innovative and practical nano-biocatalyst for biomass conversion. The conjugated enzymes showed an enhanced optimum temperature (∼ 10 °C), improved thermal stability, and shifted optimum pH toward alkaline pHs. Covalent attachment could successfully suppress the enzyme leaching and provide easy recovery/reuse of the nano-biocatalyst up to 10 cycles, with > 50% of initial activity. Application of the nano-biocatalyst in hydrolysis of rice straw and sugar beet pulp showed an increase (20-76%) in the yield of fermentable sugars compared to the free enzyme cocktails.

Synthesis of green and pure copper oxide nanoparticles using two plant resources via solid-state route and their phytotoxicity assessment.

Among the conventional methods in synthesizing nanoparticles, the methods that use biological resources, as reducing and stabilizing agents, can be considered eco-friendly methods. In this study, the leaf tissue of green tea (Camellia sinensis L.) and lavender (Lavandula anguistifolia) were utilized by the solid-state method as a one-step and low-cost method for the biosynthesis of copper oxide nanoparticles (CuO NPs). The results of the X-ray Diffraction (XRD), field emission scanning electron spectroscopy (FESEM) and transmission electron microscopy (TEM) showed that lavender is more productive in the synthesis of pure and uniform CuO NPs (50 nm). Comparing biogenic synthesized CuO NPs with chemically synthesized CuO NPs in terms of induction of phytotoxicity, exposed in treatments with concentrations of 40, 400 and 4000 µg ml-1, green CuO NPs had less inhibitory effects on the seed germination factors (i.e., germination percentage, germination rate, shoot and root length, etc.) of lettuce (Lactuca sativa L.), and tomato (Solanum lycopersicum L.) seeds. However, both green/chemically synthesized CuO NPs at their lowest concentrations (4 µg ml-1), had an effective role in root and shoot expansion of lettuce and tomato seedlings.

Application of the immobilized enzyme on magnetic graphene oxide nano-carrier as a versatile bi-functional tool for efficient removal of dye from water.

Herein, we report bi-functional applications of a novel immobilized enzyme on the modified magnetic graphene oxide (GO) for effective removal of dyes from water. The amine functionalized GO nano-carrier was covalently attached to a model enzyme (PersiManXyn1). The enzyme assays showed that the specific activities of the free and immobilized enzyme were 856.05 and 1141.1 µmolmin-1mg-1, respectively. While the free enzyme showed only 5% of its maximum activity, the immobilized PersiManXyn1 preserved more than 35% of its activity, at 90 °C. After four weeks storage, the free enzyme has been deactivated, but the immobilized enzyme retained 54% of its initial activity. The immobilized PersiManXyn1 was proficiently applied for dye removal from water using two strategies. While only pristine nano-carrier and free enzyme showed no considerable catalytic ability, the immobilized PersiManXyn1 could catalytically reduce the concentrated dye solutions within 150 s with superior reusability (94% dye removal after 15th cycle). Proficient treatment of a real textile effluent by the immobilized PersiManXyn1 approved its practical applications in the water remediation.

Nano-carriers effects on the viability and efficiency of Pseudomonas strains as phosphate solubilizing bacteria.

In order to develop nanotechnology application in the agricultural systems achieving more sustainability in the environment, we have used different nano-carriers for phosphate solubilizing bacteria. The viability and efficacy of two bacterial species; Pseudomonas putida (PP20) and Pseudomonas kilonensis (PK11) in solubilizing phosphate sources (i.e., tricalcium phosphate and hydroxyapatite) with different nano-carriers including nanoclay, natural char micro-particles (NCMPs), nanoclay + alginate, NCMPs + alginate, and natural char nano-particles (NCNPs)+alginate were investigated. Clay, talc powder, and natural char (NC) were included for comparison. The synthesized NCNPs and NCMPs were characterized using FTIR, SEM and Boehm titration analyses. The results confirmed that the chemical oxidation of pristine char made many oxygenated functional groups on the surface of tiny and spherical NCNPs (14.8 nm) which caused their effective incorporation in the matrix of alginate beads. Results of phosphate solubilizing study showed that P. kilonensis was the superior species for viability and stability of its performance on solubilizing phosphorus. The six months evaluation showed that NCNPs + alginate and nanoclay + alginate carriers at both temperatures (4 °C and 28 °C), were the proficient carriers for preserving both bacteria. The results of solubilizing phosphorus sources revealed that both bacteria solubilized tricalcium phosphate more than hydroxyapatite and PK11 showed more privilege in this regard. In addition, the solubilizing index determined after storage for 6 months at 4 °C was higher for all the carriers. Analysis of variance for phosphatase activity revealed that embedding both bacteria in nanoclay + alginate carrier guaranteed the highest phosphatase activity, even though differences between this carrier and NCNPs + alginate and NCMPs + alginate were not significant for the PK11.

Proficient dye removal from water using biogenic silver nanoparticles prepared through solid-state synthetic route.

An environmentally benign, one-pot and highly scalable method was presented to produce biogenic silver nanoparticles (Ag NPs) using the solid-state synthetic route. Four plant-derived candidate bio-reductants (i.e., Datura stramonium, Papaver orientale, Mentha piperita, and Cannabis sativa) were investigated to compare the efficiency of solid-state route and typical solution method. M. piperita was selected as the best plant resource to produce totally pure and uniform Ag NPs (average diameter of 15 nm) without any aggregation. The purity and size of biogenic Ag NPs, were tailored by adjusting the M. piperita leaf powder/silver nitrate weight ratio and temperature. The as-synthesized Ag NPs were effectively utilized as an eco-friendly nanoadsorbent in water remediation to remove a model dye (i.e., crystal violet). The key factors affecting on the sorption process (i.e., nanoadsorbent dosage, temperature, pH, dye initial concentration, and shaking time) were investigated. The pseudo-second-order kinetic model was well fitted to the sorption process and at the optimum sorption conditions, based on the Langmuir model, the adsorption capacity was found to be 704.7 mg/g. The current, cost effective and feasible method could be considered as an applicable strategy to produce green, reusable and proficient Ag NPs as nanoadsorbents for removal of dyes from contaminated water.

The Stabilizing Mechanism of Immobilized Metagenomic Xylanases on Bio-Based Hydrogels to Improve Utilization Performance: Computational and Functional Perspectives.

While polysaccharide-based superabsorbent hydrogels (SHs) have attracted increasing interest as proficient carriers in the enzyme immobilization, the nature of the favored interactions between the SHs and enzymes is still unclear. Herein, a combined experimental and computational study was employed to investigate the dominant parameters affecting on the stabilization of two metagenomic xylanases on the SHs. The thermostable enzymes (PersiXyn3 and PersiXyn4) with similar domains were screened, cloned, expressed, and purified from cattle rumen metagenome. Then, the enzymes were immobilized on the carboxymethyl cellulose-g-poly(acrylic acid-co-acrylamide) hydrogel which resulted in increasing their activity and stability. The carboxymethyl cellulose (CMC)-based characteristic of the hydrogel provided high numbers of H-bondings/ionic bridges, causing an improvement in the stability, hydrolysis performance, and reusability of the immobilized enzymes. More specifically, enzyme immobilization resulted in ∼40% increase in the content of the reducing sugars released after treatment of paper pulp. After 16 reuse cycles, the immobilized PersiXyn4 displayed 35.9% activity, but the immobilized PersiXyn3 retained just 8.2% of its initial activity. The comparative investigations illustrated that a higher number of positively charged amino acids in the binding site of the enzyme provided stronger electrostatic attractions between it and negative functionalities of the hydrogel. This was suggested as the main reason for the higher affinity of PersiXyn4 toward hydrogel and explained the better hydrolysis performance and reusability of the immobilized PersiXyn4 on the SH. These findings are essential for designing novel innovative SH carriers and the successful engineering of optimal enzyme assemblies through the prediction of the immobilized enzyme's stabilities.

Application of cellulose nanocrystals prepared from agricultural wastes for synthesis of starch-based hydrogel nanocomposites: Efficient and selective nanoadsorbent for removal of cationic dyes from water.

A novel and bio-based hydrogel nanocomposite were developed using reinforcement of starch grafted copolymers of 2-acrylamido-2methyl propane sulfonate and acrylic acid (starch-g-(AMPS-co-AA)) hydrogel with magnetite-functionalized cellulose nanocrystals (MCNCs). The MCNCs-hydrogel was utilized as a proficient and environmentally benign nanoadsorbent for removal of cationic dyes with great capacity and selectivity. Sugar-beet pulp (SBP) was exploited for CNCs production through acid hydrolysis of enzymatic-mediated SBP. Fe3O4 nanoparticles were anchored on CNCs to synthesize MCNCs as the nanofiller of MCNCs/starch-g-(AMPS-co-AA) hydrogel. The synthesis of CNCs, MCNCs and hydrogel nanocomposite were confirmed using FESEM, FTIR, VSM and TGA analyses. Hydrogel nanocomposite showed excellent and reusable capacity for specific adsorption of cationic dyes. The effects of nanoadsorbent dosage, time, pH, dye initial concentration, and temperature on the adsorption were scrutinized for two model cationic dyes (crystal violet (CV) and methylene blue (MB). Adsorption capacities for CV and MB were 2500.0 mg/g and 1428.6 mg/g, respectively.

Intraperitoneal Injection of Graphene Oxide Nanoparticle Accelerates Stem Cell Therapy Effects on Acute Kidney Injury.

PURPOSE: Graphene-based nanostructures have shown some degree of stem cell protection against cell death. Acute kidney injury (AKI) is a major cause of mortality in hospitalized patients. Here, graphene oxide (GO) was used to improve the efficacy of bone marrow-derived mesenchymal stem cells (MSCs) in the treatment of AKI induced by cisplatin, a chemotherapy medication used to treat a number of cancers. MATERIALS AND METHODS: Cisplatin-induced AKI was modeled in male rats. Intraperitoneal injection of MSCs mixed with GO, synthesized by graphite powder, H2SO4, and KMnO4 was administered in modeled animals. Biochemical analysis of serum and histological and immunohistochemical (IHC) staining of kidney tissue samples were determined. RESULTS: Administration of GO nanoparticles suspended in MSCs reduced serum levels of creatinine (Cr) and blood urea nitrogen (BUN) in cisplatin-induced AKI in the experimental group compared to the control group. Histopathological evaluation also showed an improvement of morphological alterations of kidney, such as cellular proliferation, apoptosis and necrosis, cyst formation and intratubular debris in the experimental group compared to the control group. Our data revealed that GO injection alone without MSCs accelerated the improvement of the kidney injury induced by cisplatin. CONCLUSION: This study demonstrated that suspended GO could enhance the efficacy of stem cells in the treatment of AKI. GO alone without stem cell accelerates the improvement of cisplatin-induced AKI.